BAIT

MYL6B

MLC1SA
myosin, light chain 6B, alkali, smooth muscle and non-muscle
Homo sapiens
PREY

IQGAP1

HUMORFA01, SAR1, p195
IQ motif containing GTPase activating protein 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The interaction of IQGAPs with calmodulin-like proteins.

Pathmanathan S, Hamilton E, Atcheson E, Timson DJ

Since their identification over 15 years ago, the IQGAP (IQ-motif-containing GTPase-activating protein) family of proteins have been implicated in a wide range of cellular processes, including cytoskeletal reorganization, cell-cell adhesion, cytokinesis and apoptosis. These processes rely on protein-protein interactions, and understanding these (and how they influence one another) is critical in determining how the IQGAPs function. A key group of interactions ... [more]

Biochem. Soc. Trans. Apr. 01, 2011; 39(2);694-9 [Pubmed: 21428964]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IQGAP1 MYL6B
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
1457676

Curated By

  • BioGRID