YWHAZ
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DAF-16
Gene Ontology Biological Process
- aging [IDA, IMP]
- cellular response to DNA damage stimulus [IDA]
- cellular response to heat [IDA]
- dauer entry [IMP]
- dauer larval development [IGI]
- defense response [IMP]
- defense response to Gram-negative bacterium [IMP]
- determination of adult lifespan [IDA, IGI, IMP]
- innate immune response [IMP]
- insulin receptor signaling pathway [IDA, TAS]
- lipid metabolic process [IMP]
- muscle tissue development [IMP]
- negative regulation of multicellular organism growth [IMP]
- nematode larval development [IMP]
- positive regulation of adaptive immune response [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- regulation of DNA-templated transcription in response to stress [IMP]
- regulation of cell cycle process [IEP]
- regulation of lipid biosynthetic process [IMP]
- regulation of transcription, DNA-templated [IDA]
- response to UV [IDA]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Phosphatidylinositol 3-kinase signaling inhibits DAF-16 DNA binding and function via 14-3-3-dependent and 14-3-3-independent pathways.
In Caenorhabditis elegans, an insulin-like signaling pathway to phosphatidylinositol 3-kinase (PI 3-kinase) and AKT negatively regulates the activity of DAF-16, a Forkhead transcription factor. We show that in mammalian cells, C. elegans DAF-16 is a direct target of AKT and that AKT phosphorylation generates 14-3-3 binding sites and regulates the nuclear/cytoplasmic distribution of DAF-16 as previously shown for its mammalian ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| YWHAZ DAF-16 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID