CREBBP
Gene Ontology Biological Process
- N-terminal peptidyl-lysine acetylation [ISO]
- cell proliferation [ISO]
- germ-line stem cell maintenance [IMP]
- histone acetylation [IDA, ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of G1/S transition of mitotic cell cycle [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- MRF binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [ISO]
- acetyltransferase activity [IDA, ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA]
- core promoter proximal region sequence-specific DNA binding [ISO]
- histone acetyltransferase activity [IDA, ISO]
- p53 binding [ISO]
- peroxisome proliferator activated receptor binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription coactivator activity [ISO, TAS]
- transcription factor binding [IPI, ISO]
- DNA binding [IDA]
- MRF binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [ISO]
- acetyltransferase activity [IDA, ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA]
- core promoter proximal region sequence-specific DNA binding [ISO]
- histone acetyltransferase activity [IDA, ISO]
- p53 binding [ISO]
- peroxisome proliferator activated receptor binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription coactivator activity [ISO, TAS]
- transcription factor binding [IPI, ISO]
Gene Ontology Cellular Component
MITF
Gene Ontology Biological Process
- Wnt signaling pathway [IDA]
- bone remodeling [IMP]
- camera-type eye development [IGI]
- canonical Wnt signaling pathway involved in negative regulation of apoptotic process [IDA]
- cell differentiation [IMP]
- cell fate commitment [IMP]
- melanocyte differentiation [IGI, IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- osteoclast differentiation [IMP]
- pigmentation [IGI, IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO, ISS]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- protein complex assembly [ISO]
- regulation of cell proliferation [IDA]
- regulation of gene expression [IMP]
- regulation of osteoclast differentiation [IGI, IMP]
- regulation of transcription, DNA-templated [IDA]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, ISO]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- chromatin binding [IDA]
- sequence-specific DNA binding [ISA]
- sequence-specific DNA binding transcription factor activity [IDA]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, ISO]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- chromatin binding [IDA]
- sequence-specific DNA binding [ISA]
- sequence-specific DNA binding transcription factor activity [IDA]
Gene Ontology Cellular Component
- nucleus [IC, IDA]
- protein complex [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
CBP/p300 as a co-factor for the Microphthalmia transcription factor.
The Microphthalmia basic-Helix-Loop-Helix-Leucine Zipper (bHLH-LZ) transcription factor (Mi) plays a crucial role in the genesis of melanocytes; mice deficient for a functional (Microphthalmia) gene product lack all pigment cells. We show here that the Mi activation domain resides N-terminal to the DNA-binding domain and that as little as 18 amino acids are sufficient to mediate transcription activation. The minimal activation ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CREBBP MITF | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| MITF CREBBP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID