BAIT
SLI15
L000003320, YBR156C
Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p
GO Process (3)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
RFA3
RPA14, RPA3, L000001622, YJL173C
Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress
GO Process (14)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
- DNA repair [IMP]
- DNA replication [IMP]
- DNA topological change [IDA]
- DNA unwinding involved in DNA replication [IDA]
- double-strand break repair via homologous recombination [IGI]
- establishment of protein localization [IPI]
- heteroduplex formation [IDA]
- mitotic recombination [IPI]
- nucleotide-excision repair [IDA]
- protein ubiquitination [IPI]
- reciprocal meiotic recombination [IPI]
- telomere maintenance via recombination [IPI]
- telomere maintenance via telomerase [IPI]
- telomere maintenance via telomere lengthening [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systematic exploration of essential yeast gene function with temperature-sensitive mutants.
Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 (∼45%) of the 1,101 essential yeast genes, with ∼30% of the genes represented by multiple alleles. All of the alleles are integrated into their native genomic locus in the S288C common reference strain and are linked to ... [more]
Unknown Mar. 27, 2011; 0(0); [Pubmed: 21441928]
Throughput
- High Throughput
Curated By
- BioGRID