BAIT
FAR1
cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1, L000000600, YJL157C
CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites
GO Process (3)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
STE7
mitogen-activated protein kinase kinase STE7, L000002117, YDL159W
Signal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway
GO Process (8)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- MAPK cascade involved in cell wall organization or biogenesis [IGI]
- MAPK cascade involved in conjugation with cellular fusion [IDA, IPI]
- activation of MAPK activity involved in conjugation with cellular fusion [IDA, IMP]
- invasive growth in response to glucose limitation [IGI, IMP]
- protein phosphorylation [IDA, IMP]
- pseudohyphal growth [IMP]
- regulation of transposition, RNA-mediated [IMP]
- signal transduction involved in filamentous growth [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Phenotypic Suppression
A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p.
In Saccharomyces cerevisiae, mating pheromones activate two MAP kinases (MAPKs), Fus3p and Kss1p, to induce G1 arrest prior to mating. Fus3p is known to promote G1 arrest by activating Far1p, which inhibits three Clnp/Cdc28p kinases. To analyze the contribution of Fus3p and Kss1p to G1 arrest that is independent of Far1p, we constructed far1 CLN strains that undergo G1 arrest ... [more]
Genetics Mar. 01, 1999; 151(3);989-1004 [Pubmed: 10049917]
Throughput
- Low Throughput
Ontology Terms
- phenotype: pheromone sensitivity (APO:0000037)
Additional Notes
- deletion of Ste7 blocks the pheromone sensitivity seen in a Far1/Ste11 double mutant
- genetic complex
Curated By
- BioGRID