BAIT

SSN8

CNC1, GIG3, NUT9, RYE2, SRB11, UME3, CycC, L000002796, YNL025C
Cyclin-like component of the RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; forms a kinase-cyclin pair in the RNAPII holoenzyme with Ssn3p; required for both entry into and execution of the meiotic program; involved in glucose repression and telomere maintenance; cyclin homolog 35% identical to human cyclin C
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

GIS4

L000004864, YML006C
CAAX box containing protein of unknown function; proposed to be involved in the RAS/cAMP signaling pathway
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells.

Balciunas D, Ronne H

Cyclin C and the cyclin C-dependent protein kinase are associated with the RNA polymerase II Mediator complex, which regulates initiation of transcription in response to signals from activators and repressors bound to upstream promoter elements. Disruption of the corresponding genes, SRB11 and SRB10, in budding yeast causes a reduction in expression of the GAL genes, which is particularly pronounced in ... [more]

Mol. Gen. Genet. Dec. 01, 1999; 262(4);589-99 [Pubmed: 10628841]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: utilization of carbon source (APO:0000098)

Additional Notes

  • Gis4 rescues the growth of an Snf1/Mig1/Srb11 triple mutant on galactose
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SSN8 GIS4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.998BioGRID
-

Curated By

  • BioGRID