BAIT

SHO1

SSU81, osmosensor SHO1, L000002632, L000002823, YER118C
Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
Saccharomyces cerevisiae (S288c)
PREY

PTP2

tyrosine protein phosphatase PTP2, L000001526, YOR208W
Nuclear phosphotyrosine-specific phosphatase involved in osmosensing; involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; with Msg5p co-regulates the calcium signaling pathway
Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Prediction of putative protein interactions through evolutionary analysis of osmotic stress response in the model yeast Saccharomyces cerevisae.

Thorne TW, Ho HL, Huvet M, Haynes K, Stumpf MP

The osmotic stress response signalling pathway of the model yeast Saccharomyces cerevisae is crucial for the survival of cells under osmotic stress, and is preserved to varying degrees in other related fungal species. We apply a method for inference of ancestral states of characteristics over a phylogeny to 17 fungal species to infer the maximum likelihood estimate of presence or ... [more]

Fungal Genet. Biol. May. 01, 2011; 48(5);504-11 [Pubmed: 21193057]

Throughput

  • Low Throughput

Additional Notes

  • Directionality not explicit in paper

Curated By

  • BioGRID