BAIT

KIP1

CIN9, L000000907, YBL063W
Kinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
Saccharomyces cerevisiae (S288c)
PREY

CDC14

OAF3, phosphoprotein phosphatase CDC14, L000000254, YFR028C
Protein phosphatase required for mitotic exit; required for rDNA segregation, cytokinesis, meiosis I spindle disassembly, and environmental stress response; maintained in nucleolus by Cdc55p in early meiosis until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to effect a decrease in CDK/B-cyclin activity and mitotic exit; sequestered in metaphase II, then released again upon entry into anaphase II
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Cdc14 inhibition by the spindle assembly checkpoint prevents unscheduled centrosome separation in budding yeast.

Chiroli E, Rancati G, Catusi I, Lucchini G, Piatti S

The spindle assembly checkpoint (SAC) is an evolutionarily conserved surveillance mechanism that delays anaphase onset and mitotic exit in response to the lack of kinetochore attachment. The target of the SAC is the E3 ubiquitin ligase anaphase-promoting complex (APC) bound to its Cdc20 activator. The Cdc20/APC complex is in turn required for sister chromatid separation and mitotic exit through ubiquitin-mediated ... [more]

Mol. Biol. Cell May. 01, 2009; 20(10);2626-37 [Pubmed: 19339280]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chromosome segregation (APO:0000208)

Additional Notes

  • genetic complex
  • overexpression of Cdc14 increases the chromatid segregation seen in a Cin8/Kip1 double mutant

Curated By

  • BioGRID