BAIT
DPB11
protein kinase activating protein DPB11, L000003001, YJL090C
DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
GO Process (11)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- DNA replication checkpoint [IMP]
- DNA replication initiation [IMP]
- double-strand break repair via break-induced replication [IMP]
- lagging strand elongation [TAS]
- leading strand elongation [TAS]
- mating type switching [IMP]
- mismatch repair [NAS]
- mitotic G2 DNA damage checkpoint [IGI]
- nucleotide-excision repair [TAS]
- positive regulation of protein phosphorylation [IDA]
- recombinational repair [IDA, IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
RFX1
CRT1, L000004129, YLR176C
Major transcriptional repressor of DNA-damage-regulated genes; recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Rescue
A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.
Publication
Sensing of Replication Stress and Mec1 Activation Act through Two Independent Pathways Involving the 9-1-1 Complex and DNA Polymerase ε.
Following DNA damage or replication stress, budding yeast cells activate the Rad53 checkpoint kinase, promoting genome stability in these challenging conditions. The DNA damage and replication checkpoint pathways are partially overlapping, sharing several factors, but are also differentiated at various levels. The upstream kinase Mec1 is required to activate both signaling cascades together with the 9-1-1 PCNA-like complex and the ... [more]
PLoS Genet. Mar. 01, 2011; 7(3);e1002022 [Pubmed: 21436894]
Throughput
- Low Throughput
Ontology Terms
- resistance to chemicals (APO:0000087)
Additional Notes
- Deletion of crt1 allows the partial recovery of ddc1 dpb11 double mutants in HU.
Curated By
- BioGRID