BAIT
AXIN1
AI316800, Axin, Fu, Kb, Ki, fused, kinky, knobbly
axin 1
GO Process (53)
GO Function (19)
GO Component (14)
Gene Ontology Biological Process
- Wnt signaling pathway [IDA, IPI, ISO]
- Wnt signaling pathway involved in somitogenesis [IBA]
- Wnt-activated signaling pathway involved in forebrain neuron fate commitment [IBA]
- activation of JUN kinase activity [IBA]
- activation of protein kinase activity [ISO]
- adult walking behavior [IMP]
- axial mesoderm development [IMP]
- axial mesoderm formation [IMP]
- canonical Wnt signaling pathway [IDA, IMP]
- canonical Wnt signaling pathway involved in neural plate anterior/posterior pattern formation [IBA]
- cell death [IBA]
- cellular protein complex assembly [ISO]
- cellular response to organic cyclic compound [IBA]
- cytoplasmic microtubule organization [IGI]
- determination of left/right symmetry [IBA]
- dorsal/ventral axis specification [IDA, ISO]
- dorsal/ventral pattern formation [IMP, ISO]
- embryonic eye morphogenesis [IBA]
- embryonic skeletal joint morphogenesis [IMP]
- erythrocyte homeostasis [IMP]
- forebrain anterior/posterior pattern specification [IBA]
- genetic imprinting [IMP]
- hemoglobin metabolic process [IMP]
- in utero embryonic development [IGI, IMP]
- muscle cell development [IBA]
- negative regulation of Wnt signaling pathway [IDA, ISO]
- negative regulation of canonical Wnt signaling pathway [IDA]
- negative regulation of fat cell differentiation [IDA]
- negative regulation of protein metabolic process [IDA]
- negative regulation of transcription elongation from RNA polymerase II promoter [IDA]
- neurological system process [IMP]
- nucleocytoplasmic transport [IDA]
- olfactory placode formation [IBA]
- optic placode formation [IBA]
- positive regulation of GTPase activity [IBA]
- positive regulation of JNK cascade [IMP]
- positive regulation of JUN kinase activity [IDA, IMP, ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein kinase activity [ISO]
- positive regulation of protein phosphorylation [IDA, ISO]
- positive regulation of protein ubiquitination [IDA, IGI, IMP]
- positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA, ISO]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation of transforming growth factor beta receptor signaling pathway [IDA, IGI, IMP]
- positive regulation of ubiquitin-protein transferase activity [IMP, ISO]
- post-anal tail morphogenesis [IMP]
- protein catabolic process [IDA]
- protein homooligomerization [IDA, ISO]
- protein polyubiquitination [IDA]
- regulation of catenin import into nucleus [IDA]
- regulation of protein phosphorylation [IGI]
- sensory perception of sound [IMP]
Gene Ontology Molecular Function- GTPase activator activity [IBA]
- I-SMAD binding [IDA, IPI, ISO]
- R-SMAD binding [IPI]
- SMAD binding [ISO]
- armadillo repeat domain binding [IBA, ISO]
- beta-catenin binding [IMP, IPI, ISO]
- enzyme binding [ISO]
- identical protein binding [IPI, ISO]
- p53 binding [IPI]
- protein C-terminus binding [IDA, IPI]
- protein binding [IPI]
- protein complex scaffold [IMP, ISO]
- protein domain specific binding [IPI, ISO]
- protein homodimerization activity [IPI]
- protein kinase binding [IPI, ISO]
- protein self-association [IPI]
- receptor binding [ISO]
- signal transducer activity [ISO]
- ubiquitin protein ligase binding [IPI, ISO]
- GTPase activator activity [IBA]
- I-SMAD binding [IDA, IPI, ISO]
- R-SMAD binding [IPI]
- SMAD binding [ISO]
- armadillo repeat domain binding [IBA, ISO]
- beta-catenin binding [IMP, IPI, ISO]
- enzyme binding [ISO]
- identical protein binding [IPI, ISO]
- p53 binding [IPI]
- protein C-terminus binding [IDA, IPI]
- protein binding [IPI]
- protein complex scaffold [IMP, ISO]
- protein domain specific binding [IPI, ISO]
- protein homodimerization activity [IPI]
- protein kinase binding [IPI, ISO]
- protein self-association [IPI]
- receptor binding [ISO]
- signal transducer activity [ISO]
- ubiquitin protein ligase binding [IPI, ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- beta-catenin destruction complex [IDA, ISO]
- cell cortex [IDA]
- cell periphery [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic membrane-bounded vesicle [IDA]
- cytoplasmic microtubule [IDA]
- cytoplasmic vesicle [ISO]
- lateral plasma membrane [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IBA, ISO]
- protein complex [IDA]
Mus musculus
PREY
PPP2R1A
6330556D22Rik, PP2A, PR65
protein phosphatase 2, regulatory subunit A, alpha
GO Process (3)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Identification of a domain of Axin that binds to the serine/threonine protein phosphatase 2A and a self-binding domain.
Axin is a negative regulator of embryonic axis formation in vertebrates, which acts through a Wnt signal transduction pathway involving the serine/threonine kinase GSK-3 and beta-catenin. Axin has been shown to have distinct binding sites for GSK-3 and beta-catenin and to promote the phosphorylation of beta-catenin and its consequent degradation. This provides an explanation for the ability of Axin to ... [more]
J. Biol. Chem. Feb. 05, 1999; 274(6);3439-45 [Pubmed: 9920888]
Throughput
- Low Throughput
Curated By
- BioGRID