AI316800, Axin, Fu, Kb, Ki, fused, kinky, knobbly
axin 1
GO Process (53)
GO Function (19)
GO Component (14)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.


Identification of a domain of Axin that binds to the serine/threonine protein phosphatase 2A and a self-binding domain.

Hsu W, Zeng L, Costantini F

Axin is a negative regulator of embryonic axis formation in vertebrates, which acts through a Wnt signal transduction pathway involving the serine/threonine kinase GSK-3 and beta-catenin. Axin has been shown to have distinct binding sites for GSK-3 and beta-catenin and to promote the phosphorylation of beta-catenin and its consequent degradation. This provides an explanation for the ability of Axin to ... [more]

J. Biol. Chem. Feb. 05, 1999; 274(6);3439-45 [Pubmed: 9920888]


  • Low Throughput

Curated By

  • BioGRID