BAIT

PPP2CA

PP2A, R75353, RP23-46N3.2
protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
GO Process (19)
GO Function (15)
GO Component (9)
Mus musculus

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of a domain of Axin that binds to the serine/threonine protein phosphatase 2A and a self-binding domain.

Hsu W, Zeng L, Costantini F

Axin is a negative regulator of embryonic axis formation in vertebrates, which acts through a Wnt signal transduction pathway involving the serine/threonine kinase GSK-3 and beta-catenin. Axin has been shown to have distinct binding sites for GSK-3 and beta-catenin and to promote the phosphorylation of beta-catenin and its consequent degradation. This provides an explanation for the ability of Axin to ... [more]

J. Biol. Chem. Feb. 05, 1999; 274(6);3439-45 [Pubmed: 9920888]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PPP2CA PPP2R1A
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.9605BioGRID
2672454

Curated By

  • BioGRID