SCH9
Gene Ontology Biological Process
- age-dependent response to oxidative stress involved in chronological cell aging [IMP]
- positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase I promoter [IGI, IMP]
- positive regulation of transcription from RNA polymerase III promoter [IGI, IMP]
- protein phosphorylation [IMP]
- regulation of cell size [IMP]
- regulation of protein localization [IMP]
- regulation of response to osmotic stress [IMP]
- regulation of sphingolipid biosynthetic process [IMP]
- regulation of transcription from RNA polymerase II promoter in response to oxidative stress [IMP]
- replicative cell aging [IMP]
Gene Ontology Molecular Function
JJJ3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Diverse protein kinase interactions identified by protein microarrays reveal novel connections between cellular processes.
Protein kinases are key regulators of cellular processes. In spite of considerable effort, a full understanding of the pathways they participate in remains elusive. We globally investigated the proteins that interact with the majority of yeast protein kinases using protein microarrays. Eighty-five kinases were purified and used to probe yeast proteome microarrays. One-thousand-twenty-three interactions were identified, and the vast majority ... [more]
Throughput
- High Throughput
Additional Notes
- High Throughput: Proteome microarrays were used to identify proteins that interact with protein kinases.
Curated By
- BioGRID