USF1
Gene Ontology Biological Process
- carbon catabolite regulation of transcription [TAS]
- cellular response to insulin stimulus [IDA]
- glucose homeostasis [TAS]
- late viral transcription [IDA]
- lipid homeostasis [ISS]
- negative regulation of fibrinolysis [IC]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter by glucose [IMP, ISS]
- regulation of transcription from RNA polymerase II promoter [TAS]
- regulation of transcription from RNA polymerase II promoter by glucose [IC]
- response to UV [ISS]
- response to hypoxia [IMP]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA, IMP]
- bHLH transcription factor binding [IPI]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI, TAS]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA, IMP]
- bHLH transcription factor binding [IPI]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI, TAS]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
Gene Ontology Cellular Component
USF2
Gene Ontology Biological Process
- late viral transcription [IC]
- lipid homeostasis [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter by glucose [IMP, ISS]
- regulation of transcription from RNA polymerase II promoter by glucose [IC]
- transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IMP]
- bHLH transcription factor binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IMP]
- bHLH transcription factor binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier.
The insulator element at the 5' end of the chicken beta-globin locus acts as a barrier, protecting transgenes against silencing effects of adjacent heterochromatin. We showed earlier that the transcription factor USF1 binds within the insulator and that this site is important for generating in adjacent nucleosomes histone modifications associated with active chromatin and, by inference, with barrier function. To ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| USF2 USF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.442 | BioGRID | 241795 | |
| USF1 USF2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1448065 | |
| USF1 USF2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2216962 | |
| USF1 USF2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3120535 | |
| USF1 USF2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 676744 | |
| USF1 USF2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USF2 USF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USF1 USF2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USF1 USF2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USF1 USF2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USF1 USF2 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 885846 | |
| USF1 USF2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID