An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The DNA damage response mediator MDC1 directly interacts with the anaphase-promoting complex/cyclosome.

Coster G, Hayouka Z, Argaman L, Strauss C, Friedler A, Brandeis M, Goldberg M

MDC1 (NFBD1), a mediator of the cellular response to DNA damage, plays an important role in checkpoint activation and DNA repair. Here we identified a cross-talk between the DNA damage response and cell cycle regulation. We discovered that MDC1 binds the anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that controls the cell cycle. The interaction is direct and is mediated ... [more]

J. Biol. Chem. Nov. 02, 2007; 282(44);32053-64 [Pubmed: 17827148]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MDC1 ANAPC1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Woods NT (2012)	High	-	BioGRID	-
ANAPC1 MDC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Townsend K (2009)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MDC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

FHA domain binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

ANAPC1