An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Charting the landscape of tandem BRCT domain-mediated protein interactions.

Woods NT, Mesquita RD, Sweet M, Carvalho MA, Li X, Liu Y, Nguyen H, Thomas CE, Iversen ES, Marsillac S, Karchin R, Koomen J, Monteiro AN

Eukaryotic cells have evolved an intricate system to resolve DNA damage to prevent its transmission to daughter cells. This system, collectively known as the DNA damage response (DDR) network, includes many proteins that detect DNA damage, promote repair, and coordinate progression through the cell cycle. Because defects in this network can lead to cancer, this network constitutes a barrier against ... [more]

Sci Signal Sep. 20, 2012; 5(242);rs6 [Pubmed: 22990118]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MDC1 ANAPC1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Coster G (2007)	Low	-	BioGRID	-
ANAPC1 MDC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Townsend K (2009)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MDC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

FHA domain binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

ANAPC1