RNH35, Rnh2A, L000004047, YNL072W
Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)


mismatch repair protein MSH3, L000001191, YCR092C
Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.


Mismatch repair-independent tandem repeat sequence instability resulting from ribonucleotide incorporation by DNA polymerase É›

Clark AB, Lujan SA, Kissling GE, Kunkel TA

During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutator allele of Pol ɛ (pol2-M644G), failure to ... [more]

Unknown Mar. 15, 2011; 0(0); [Pubmed: 21414850]


  • Low Throughput

Ontology Terms

  • phenotype: mutation frequency (APO:0000198)

Additional Notes

  • deletion of msh3 enhances mutation rates in an rnh201/pol2/msh6 triple mutant
  • genetic complex

Curated By

  • BioGRID