AA673258, SIR2L1, Sir2, Sir2a, Sir2alpha
sirtuin 1
GO Process (101)
GO Function (21)
GO Component (18)

Gene Ontology Biological Process

Mus musculus


v-rel reticuloendotheliosis viral oncogene homolog A (avian)
GO Process (41)
GO Function (25)
GO Component (8)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.


The type III histone deacetylase Sirt1 suppresses p300- mediated histone H3 Lysine 56 acetylation at Bclaf1 promoter to inhibit T cell activation.

Kong S, Kim SJ, Sandal B, Lee SM, Gao B, Zhang DD, Fang D

The NAD-dependent histone deacetylase Sirt1 is a negative regulator of T cell activation. Here we report that Sirt1 inhibits T cell activation by suppressing the transcription of Bcl2-associated factor 1 (Bclaf1), a protein required for T cell activation. Sirt1-null T cells have increased acetylation of the histone 3 lysine 56 residue (H3K56) at the Bclaf1 promoter, as well as increased ... [more]

Unknown Mar. 22, 2011; 0(0); [Pubmed: 21454709]


  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.


Curated By

  • BioGRID