MAL3
Gene Ontology Biological Process
- attachment of mitotic spindle microtubules to kinetochore [IGI, IMP]
- cellular protein localization [IMP]
- dynein-driven meiotic oscillatory nuclear movement [IMP]
- gamma-tubulin complex localization [IMP]
- karyogamy [IMP]
- microtubule polymerization [IMP]
- microtubule-based movement [IMP]
- mitotic spindle stabilization [IGI]
- nuclear migration involved in conjugation with cellular fusion [IMP]
- positive regulation of ATPase activity [IDA]
- protein localization to microtubule [IDA, IMP]
- regulation of filamentous growth [IMP]
- spindle assembly involved in mitosis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ALP14
Gene Ontology Biological Process
- attachment of mitotic spindle microtubules to kinetochore [IDA]
- cytoplasmic microtubule organization [IMP]
- establishment or maintenance of cell polarity regulating cell shape [IMP]
- microtubule cytoskeleton organization [IMP]
- mitotic sister chromatid segregation [IMP]
- spindle assembly involved in mitosis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway ... [more]
Quantitative Score
- -15.3579 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.0 for positive interactions (epistatic or suppressor interactions) and S score < -2.5 for negative interactions (synthetic sick/lethal interactions).
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ALP14 MAL3 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 664495 | |
MAL3 ALP14 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 800005 |
Curated By
- BioGRID