BAIT
CLB6
B-type cyclin CLB6, L000000354, YGR109C
B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
CDC7
LSD6, SAS1, serine/threonine protein kinase CDC7, L000000247, YDL017W
DDK (Dbf4-dependent kinase) catalytic subunit; required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p
GO Process (9)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- DNA replication initiation [IMP]
- double-strand break repair via break-induced replication [IMP]
- negative regulation of exit from mitosis [IPI]
- peptidyl-serine phosphorylation [IDA]
- positive regulation of meiosis I [IGI]
- positive regulation of meiotic DNA double-strand break formation [IGI]
- premeiotic DNA replication [IMP]
- protein phosphorylation [IMP]
- regulation of chromatin silencing at telomere [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Dosage Lethality
A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.
Publication
Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation of yeast Mcm4 N-terminal domain.
Cyclin-dependent (CDK) and Dbf4-dependent (DDK) kinases trigger DNA replication in all eukaryotes, but how these kinases cooperate to regulate DNA synthesis is largely unknown. Here, we show that budding yeast Mcm4 is phosphorylated in vivo during S phase in a manner dependent on the presence of five CDK phosphoacceptor residues within the N-terminal domain of Mcm4. Mutation to alanine of ... [more]
Mol. Biol. Cell May. 01, 2008; 19(5);2267-77 [Pubmed: 18321994]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- Overexpression of DBF4 and CDC7 is lethal in a clb5 clb6 background.
- genetic complex
Curated By
- BioGRID