KLF5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RARA
Gene Ontology Biological Process
- apoptotic cell clearance [IMP]
- cellular response to estrogen stimulus [IDA]
- cellular response to retinoic acid [IDA]
- gene expression [TAS]
- intracellular estrogen receptor signaling pathway [IDA]
- negative regulation of granulocyte differentiation [IDA]
- negative regulation of interferon-gamma production [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of tumor necrosis factor production [IDA]
- positive regulation of T-helper 2 cell differentiation [IDA]
- positive regulation of binding [IMP]
- positive regulation of cell cycle [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of interleukin-13 production [IDA]
- positive regulation of interleukin-4 production [IDA]
- positive regulation of interleukin-5 production [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- protein phosphorylation [IMP]
- response to estradiol [IDA]
- response to retinoic acid [IMP]
- retinoic acid receptor signaling pathway [IMP]
- signal transduction [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- chromatin DNA binding [IDA]
- enzyme binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
- protein kinase A binding [IDA]
- protein kinase B binding [IPI]
- receptor binding [IDA]
- retinoic acid binding [IDA]
- retinoic acid receptor activity [IDA]
- retinoic acid-responsive element binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA, IMP]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI]
- chromatin DNA binding [IDA]
- enzyme binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
- protein kinase A binding [IDA]
- protein kinase B binding [IPI]
- receptor binding [IDA]
- retinoic acid binding [IDA]
- retinoic acid receptor activity [IDA]
- retinoic acid-responsive element binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA, IMP]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
HDAC2 phosphorylation-dependent Klf5 deacetylation and RARα acetylation induced by RAR agonist switch the transcription regulatory programs of p21 in VSMCs.
Abnormal proliferation of vascular smooth muscle cells (VSMCs) occurs in hypertension, atherosclerosis and restenosis after angioplasty, leading to pathophysiological vascular remodeling. As an important growth arrest gene, p21 plays critical roles in vascular remodeling. Regulation of p21 expression by retinoic acid receptor (RAR) and its ligand has important implications for control of pathological vascular remodeling. Nevertheless, the mechanism of RAR-mediated ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RARA KLF5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| KLF5 RARA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RARA KLF5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RARA KLF5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RARA KLF5 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID