EXO1
Gene Ontology Biological Process
Gene Ontology Molecular Function- 5'-3' exodeoxyribonuclease activity [IDA]
- 5'-3' exonuclease activity [ISS]
- DNA binding [IDA]
- RNA-DNA hybrid ribonuclease activity [TAS]
- double-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
- exonuclease activity [TAS]
- flap endonuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
- 5'-3' exodeoxyribonuclease activity [IDA]
- 5'-3' exonuclease activity [ISS]
- DNA binding [IDA]
- RNA-DNA hybrid ribonuclease activity [TAS]
- double-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
- exonuclease activity [TAS]
- flap endonuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA 5'-3' exodeoxyribonuclease activity [IDA]
Gene Ontology Cellular Component
- nucleoplasm [IDA]
- nucleus [IDA]
YWHAB
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [TAS]
- RNA metabolic process [TAS]
- Ras protein signal transduction [TAS]
- activation of MAPKK activity [TAS]
- apoptotic process [TAS]
- axon guidance [TAS]
- cytoplasmic sequestering of protein [IDA]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- hippo signaling [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- negative regulation of protein dephosphorylation [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of catalytic activity [IDA]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
14-3-3 proteins regulate exonuclease 1-dependent processing of stalled replication forks.
Replication fork integrity, which is essential for the maintenance of genome stability, is monitored by checkpoint-mediated phosphorylation events. 14-3-3 proteins are able to bind phosphorylated proteins and were shown to play an undefined role under DNA replication stress. Exonuclease 1 (Exo1) processes stalled replication forks in checkpoint-defective yeast cells. We now identify 14-3-3 proteins as in vivo interaction partners of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| YWHAB EXO1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3375420 | |
| YWHAB EXO1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.93 | BioGRID | - | |
| YWHAB EXO1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| YWHAB EXO1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3536091 | |
| YWHAB EXO1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| YWHAB EXO1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID