BAIT

KTN1

CG1, KNT, MU-RMS-40.19
kinectin 1 (kinesin receptor)
GO Process (0)
GO Function (1)
GO Component (4)
Homo sapiens
PREY

EEF1D

EF-1D, EF1D, FP1047, UNQ601
eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Kinectin anchors the translation elongation factor-1 delta to the endoplasmic reticulum.

Ong LL, Er CP, Ho A, Aung MT, Yu H

Kinectin has been proposed to be a membrane anchor for kinesin on intracellular organelles. A kinectin isoform that lacks a major portion of the kinesin-binding domain does not bind kinesin but interacts with another resident of the endoplasmic reticulum, the translation elongation factor-1 delta (EF-1 delta). This was shown by yeast two-hybrid analysis and a number of in vitro and ... [more]

J. Biol. Chem. Aug. 22, 2003; 278(34);32115-23 [Pubmed: 12773547]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EEF1D KTN1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9978BioGRID
2234842
KTN1 EEF1D
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
KTN1 EEF1D
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID