BAIT
SIN3
CPE1, GAM2, RPD1, SDI1, SDS16, UME4, transcriptional regulator SIN3, L000001695, YOL004W
Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
GO Process (13)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- double-strand break repair via nonhomologous end joining [IMP]
- histone deacetylation [IMP]
- negative regulation of chromatin silencing at rDNA [IMP]
- negative regulation of chromatin silencing at silent mating-type cassette [IMP]
- negative regulation of chromatin silencing at telomere [IMP]
- negative regulation of transcription during meiosis [IMP]
- negative regulation of transcription from RNA polymerase I promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]
- regulation of DNA-dependent DNA replication initiation [IMP]
- regulation of transcription involved in G2/M transition of mitotic cell cycle [IGI]
- transfer RNA gene-mediated silencing [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
CLB3
B-type cyclin CLB3, L000000351, YDL155W
B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress; CLB3 has a paralog, CLB4, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Rewiring of genetic networks in response to DNA damage.
Although cellular behaviors are dynamic, the networks that govern these behaviors have been mapped primarily as static snapshots. Using an approach called differential epistasis mapping, we have discovered widespread changes in genetic interaction among yeast kinases, phosphatases, and transcription factors as the cell responds to DNA damage. Differential interactions uncover many gene functions that go undetected in static conditions. They ... [more]
Science Dec. 03, 2010; 330(6009);1385-9 [Pubmed: 21127252]
Quantitative Score
- -5.669584 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID