BAIT
PSY2
YNL201C
Subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3
GO Process (6)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IGI]
- negative regulation of DNA damage checkpoint [IMP]
- negative regulation of glucose mediated signaling pathway [IMP]
- positive regulation of double-strand break repair via nonhomologous end joining [IMP]
- protein dephosphorylation [IMP]
- signal transduction involved in meiotic recombination checkpoint [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
PTC1
CWH47, KCS2, TPD1, type 2C protein phosphatase PTC1, L000000890, L000001523, YDL006W
Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation
GO Process (5)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- inactivation of MAPK activity involved in osmosensory signaling pathway [IDA, IGI, IMP]
- mitochondrion inheritance [IMP]
- pheromone-dependent signal transduction involved in conjugation with cellular fusion [IMP]
- protein dephosphorylation [IDA]
- tRNA splicing, via endonucleolytic cleavage and ligation [IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Rewiring of genetic networks in response to DNA damage.
Although cellular behaviors are dynamic, the networks that govern these behaviors have been mapped primarily as static snapshots. Using an approach called differential epistasis mapping, we have discovered widespread changes in genetic interaction among yeast kinases, phosphatases, and transcription factors as the cell responds to DNA damage. Differential interactions uncover many gene functions that go undetected in static conditions. They ... [more]
Science Dec. 03, 2010; 330(6009);1385-9 [Pubmed: 21127252]
Quantitative Score
- -4.454245 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score >= 2.0 for positive interactions (epistatic or suppressor interactions) and S score <= -2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID