BAIT
PKH2
serine/threonine protein kinase PKH2, L000004617, YOL100W
Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication
GO Process (5)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
- MAPK cascade involved in cell wall organization or biogenesis [IGI, IMP]
- cytoplasmic mRNA processing body assembly [IGI]
- late endosome to vacuole transport via multivesicular body sorting pathway [IGI, IMP]
- protein phosphorylation [IDA]
- regulation of nuclear-transcribed mRNA poly(A) tail shortening [IGI]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
SUM1
L000003008, YDR310C
Transcriptional repressor that regulates middle-sporulation genes; required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
GO Process (4)
GO Function (4)
GO Component (1)
Gene Ontology Biological Process
- chromatin silencing at silent mating-type cassette [IGI, IMP]
- negative regulation of transcription by competitive promoter binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter during mitosis [IMP]
- positive regulation of DNA-dependent DNA replication initiation [IGI, IMP, IPI]
Gene Ontology Molecular Function- DNA replication origin binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- sequence-specific DNA binding [IDA]
- DNA replication origin binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- sequence-specific DNA binding [IDA]
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Rewiring of genetic networks in response to DNA damage.
Although cellular behaviors are dynamic, the networks that govern these behaviors have been mapped primarily as static snapshots. Using an approach called differential epistasis mapping, we have discovered widespread changes in genetic interaction among yeast kinases, phosphatases, and transcription factors as the cell responds to DNA damage. Differential interactions uncover many gene functions that go undetected in static conditions. They ... [more]
Science Dec. 03, 2010; 330(6009);1385-9 [Pubmed: 21127252]
Quantitative Score
- -2.574325 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
- phenotype: resistance to chemicals (APO:0000087)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants in MMS-treated conditions. Genetic interactions were considered significant if they had an S score >=2.0 for positive interactions (epistatic or suppressor interactions) and S score <=2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID