BAIT
PHO23
L000004300, YNL097C
Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3
GO Process (8)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
- chromatin modification [IMP, IPI, ISS]
- negative regulation of chromatin silencing at rDNA [IMP]
- negative regulation of chromatin silencing at silent mating-type cassette [IMP]
- negative regulation of chromatin silencing at telomere [IMP]
- negative regulation of transcription from RNA polymerase I promoter [IMP]
- positive regulation of invasive growth in response to glucose limitation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MSG5
tyrosine/serine/threonine protein phosphatase MSG5, L000001188, YNL053W
Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p; MSG5 has a paralog, SDP1, that arose from the whole genome duplication
GO Process (7)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
- adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [IGI, IMP]
- inactivation of MAPK activity involved in cell wall organization or biogenesis [IDA, IGI, IMP, IPI]
- inactivation of MAPK activity involved in conjugation with cellular fusion [IDA, IMP]
- protein dephosphorylation [IDA, IMP]
- regulation of MAPK export from nucleus [IMP]
- regulation of fungal-type cell wall organization [IMP]
- regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion [IBA]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Rewiring of genetic networks in response to DNA damage.
Although cellular behaviors are dynamic, the networks that govern these behaviors have been mapped primarily as static snapshots. Using an approach called differential epistasis mapping, we have discovered widespread changes in genetic interaction among yeast kinases, phosphatases, and transcription factors as the cell responds to DNA damage. Differential interactions uncover many gene functions that go undetected in static conditions. They ... [more]
Science Dec. 03, 2010; 330(6009);1385-9 [Pubmed: 21127252]
Quantitative Score
- -2.895288 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- colony size (APO:0000063)
- resistance to chemicals (APO:0000087)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants in MMS-treated conditions. Genetic interactions were considered significant if they had an S score >=2.0 for positive interactions (epistatic or suppressor interactions) and S score <=2.5 for negative interactions (synthetic sick/lethal interactions).
Curated By
- BioGRID