BAIT
RPD3
MOF6, REC3, SDI2, SDS6, histone deacetylase RPD3, L000001696, L000001603, YNL330C
Histone deacetylase, component of both the Rpd3S and Rpd3L complexes; regulates transcription, silencing, autophagy and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members; Rpd3(L) recruitment to the subtelomeric region is regulated by interaction with the arginine methyltransferase, Hmt1p
GO Process (19)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- chromatin organization involved in regulation of transcription [IMP]
- histone H3 deacetylation [IMP]
- histone H4 deacetylation [IMP]
- negative regulation of chromatin silencing at rDNA [IMP]
- negative regulation of chromatin silencing at silent mating-type cassette [IMP]
- negative regulation of chromatin silencing at telomere [IDA, IMP]
- negative regulation of reciprocal meiotic recombination [IMP]
- negative regulation of transcription during meiosis [IMP]
- negative regulation of transcription from RNA polymerase I promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI, IMP, IPI]
- positive regulation of macroautophagy [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP]
- protein localization to nucleolar rDNA repeats [IMP]
- regulation of DNA-dependent DNA replication initiation [IGI, IMP]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [IGI, IPI]
- regulation of transcription involved in G2/M transition of mitotic cell cycle [IGI]
- replicative cell aging [IMP]
- transcription elongation from RNA polymerase II promoter [IGI]
- transfer RNA gene-mediated silencing [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
YPK1
SLI2, serine/threonine protein kinase YPK1, L000002541, YKL126W
Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
GO Process (7)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- eisosome assembly [IGI]
- glycerophospholipid metabolic process [IGI, IMP]
- negative regulation of phospholipid translocation [IMP]
- negative regulation of sphingolipid biosynthesis involved in cellular sphingolipid homeostasis [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA, IMP]
- response to pheromone involved in conjugation with cellular fusion [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Rewiring of genetic networks in response to DNA damage.
Although cellular behaviors are dynamic, the networks that govern these behaviors have been mapped primarily as static snapshots. Using an approach called differential epistasis mapping, we have discovered widespread changes in genetic interaction among yeast kinases, phosphatases, and transcription factors as the cell responds to DNA damage. Differential interactions uncover many gene functions that go undetected in static conditions. They ... [more]
Science Dec. 03, 2010; 330(6009);1385-9 [Pubmed: 21127252]
Quantitative Score
- -5.662717 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
- phenotype: resistance to chemicals (APO:0000087)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants in MMS-treated conditions. Genetic interactions were considered significant if they had an S score >=2.0 for positive interactions (epistatic or suppressor interactions) and S score <=2.5 for negative interactions (synthetic sick/lethal interactions).
Related interactions
Curated By
- BioGRID