An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cohesion establishment factor, Eco1 represses transcription via association with histone demethylase, LSD1.

Choi HK, Kim BJ, Seo JH, Kang JS, Cho H, Kim ST

Accurate chromosome segregation during cell division requires physical attachment of two sister chromatids from DNA replication until mitosis, a process known as sister chromatid cohesion. Sister chromatid cohesion is mediated by a four-subunit cohesin complex that connects sister chromatids by encircling them as molecular rings. Eco1 is an essential acetyltransferase required for the establishment of sister chromatid cohesion. Eco1 acetylates ... [more]

Biochem. Biophys. Res. Commun. Apr. 16, 2010; 394(4);1063-8 [Pubmed: 20331966]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

EHMT2

Gene Ontology Biological Process

Gene Ontology Molecular Function

C2H2 zinc finger domain binding [IPI]histone methyltransferase activity (H3-K27 specific) [ISS]histone methyltransferase activity (H3-K9 specific) [ISS]histone-lysine N-methyltransferase activity [IMP]p53 binding [IPI]protein binding [IPI]protein-lysine N-methyltransferase activity [IDA]

Gene Ontology Cellular Component

ESCO1