Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.

Krebs AR, Demmers J, Karmodiya K, Chang NC, Chang AC, Tora L

The Ada-Two-A-containing (ATAC) histone acetyltransferase and Mediator coactivator complexes regulate independent and distinct steps during transcription initiation and elongation. Here, we report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. Moreover, we identify leucine zipper motif-containing protein 1 as a subunit of this meta-coactivator complex (MECO). Finally, we ... [more]

EMBO Rep. Jul. 01, 2010; 11(7);541-7 [Pubmed: 20508642]

Throughput

  • High Throughput

Curated By

  • BioGRID