BAIT

UBQ3

F15A17.270, F15A17_270, POLYUBIQUITIN 3 PROTEIN, polyubiquitin 3, AT5G03240
polyubiquitin 3
GO Process (3)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

TUB4

K9L2.12, K9L2_12, tubulin beta chain 4, AT5G44340
tubulin beta-4 chain
GO Process (0)
GO Function (1)
GO Component (9)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings.

Igawa T, Fujiwara M, Takahashi H, Sawasaki T, Endo Y, Seki M, Shinozaki K, Fukao Y, Yanagawa Y

The majority of proteins in eukaryotic cells are modified according to highly regulated mechanisms to fulfill specific functions and to achieve localization, stability, and transport. Protein ubiquitination is one of the major post-translational modifications occurring in eukaryotic cells. To obtain the proteomic dataset related to the ubiquitin (Ub)-dependent regulatory system in Arabidopsis, affinity purification with an anti-Ub antibody under native ... [more]

J. Exp. Bot. May. 12, 2009; 60(11);3067-73 [Pubmed: 19429840]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBQ3 TUB4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-

Curated By

  • BioGRID