BAIT

UBQ3

F15A17.270, F15A17_270, POLYUBIQUITIN 3 PROTEIN, polyubiquitin 3, AT5G03240
polyubiquitin 3
GO Process (3)
GO Function (0)
GO Component (2)
Arabidopsis thaliana (Columbia)
PREY

TUB4

K9L2.12, K9L2_12, tubulin beta chain 4, AT5G44340
tubulin beta-4 chain
GO Process (0)
GO Function (1)
GO Component (9)
Arabidopsis thaliana (Columbia)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of ubiquitinated proteins in Arabidopsis.

Manzano C, Abraham Z, Lopez-Torrejon G, Del Pozo JC

Ubiquitin (Ub) is a small peptide that is covalently attached to proteins in a posttranslational reaction. Ubiquitination is a precise regulatory system that is present in all eukaryotic organisms and regulates the stability, the activity, the localization and the transport of proteins. Ubiquitination involves different enzymatic activities, in which the E3 ligases catalyze the last step recruiting of the target ... [more]

Plant Mol. Biol. Sep. 01, 2008; 68(1);145-58 [Pubmed: 18535787]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBQ3 TUB4
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-

Curated By

  • BioGRID