BAIT

RIO2

protein kinase RIO2, YNL207W
Essential serine kinase involved in the processing of 20S pre-rRNA; involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
Kinome Project (Sc)
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

RPS27B

ribosomal 40S subunit protein S27B, S27e, rp61, YS20, S27B, L000002713, YHR021C
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Ribosome Assembly Factors Prevent Premature Translation Initiation by 40S Assembly Intermediates.

Strunk BS, Loucks CR, Su M, Vashisth H, Cheng S, Schilling J, Brooks CL, Karbstein K, Skiniotis G

Ribosome assembly in eukaryotes requires a total of approximately 200 essential assembly factors (AFs), and occurs through ordered events that initiate in the nucleolus and culminate in the cytoplasm. Here, we present the cryo-electron microscopy (cryo-EM) structure of a late cytoplasmic 40S ribosome assembly intermediate from Saccharomyces cerevisiae at 18 A resolution. The positions of all seven AFs bound to ... [more]

Unknown Aug. 17, 2011; 0(0); [Pubmed: 21835981]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RIO2 RPS27B
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2116BioGRID
2010973

Curated By

  • BioGRID