RAD54
Gene Ontology Biological Process
- DNA geometric change [IDA]
- chromatin remodeling [IDA]
- double-strand break repair via single-strand annealing [IMP]
- double-strand break repair via synthesis-dependent strand annealing [TAS]
- heteroduplex formation [IDA]
- positive regulation of endodeoxyribonuclease activity [IDA]
- telomere maintenance via recombination [IMP]
Gene Ontology Molecular Function
CLA4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic pathway analysis using high-resolution fitness profiling of combinatorial gene deletions.
Systematic genetic interaction studies have illuminated many cellular processes. Here we quantitatively examine genetic interactions among 26 Saccharomyces cerevisiae genes conferring resistance to the DNA-damaging agent methyl methanesulfonate (MMS), as determined by chemogenomic fitness profiling of pooled deletion strains. We constructed 650 double-deletion strains, corresponding to all pairings of these 26 deletions. The fitness of single- and double-deletion strains were ... [more]
Quantitative Score
- -0.208975 [SGA Score]
Throughput
- Low Throughput
Ontology Terms
- phenotype: growth in exponential phase (APO:0000310)
Additional Notes
- Genetic interactions were quantitatively examined among 26 genes related to DNA repair. In the presence of methyl methanesulfonate (MMS), 45 were classified as alleviating interactions (significantly positive epsilon score) and 68 were classified as aggravating interactions (significantly negative epsilon score) with a P-value < 0.01.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CLA4 RAD54 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1615 | BioGRID | 2604986 | |
| CLA4 RAD54 | Positive Genetic Positive Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores. | High | 0.1059 | BioGRID | 2605161 | |
| RAD54 CLA4 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 457252 | |
| CLA4 RAD54 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 450450 | |
| CLA4 RAD54 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 80935 |
Curated By
- BioGRID