BAIT

CLC1

SCD4, L000000355, YGR167W
Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; regulates endocytic progression; thought to regulate clathrin function; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

PAN1

DIM2, MDP3, MIP3, L000001335, YIR006C
Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p; associates with actin patches on cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates Arp2/3 complex in vitro; phosphorylation of Thr-1225 is regulated by MAPK Hog1p in response to osmotic stress; previously thought to be a subunit of poly(A) ribonuclease
Kinome Project (Sc)
GO Process (4)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Clathrin light chain directs endocytosis by influencing the binding of the yeast Hip1R homologue, Sla2, to F-actin.

Boettner DR, Friesen H, Andrews B, Lemmon SK

The role of clathrin light chain (CLC) in clathrin-mediated endocytosis is not completely understood. Previous studies have shown that the CLC N-terminus (CLC-NT) binds the Hip1/Hip1R/Sla2 family of membrane/actin binding factors and that overexpression of the CLC-NT in yeast suppresses endocytic defects of clathrin heavy chain mutants. To elucidate the mechanistic basis for this suppression, we performed Synthetic Genetic Array ... [more]

Unknown Aug. 17, 2011; 0(0); [Pubmed: 21849475]

Throughput

  • Low Throughput

Ontology Terms

  • inviable (APO:0000112)

Additional Notes

  • A clathrin CLC-NT deletion mutant (clc1-delta-19-76) was used in the experiment.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CLC1 PAN1
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
-

Curated By

  • BioGRID