BAIT

PPP2R1A

PP2A-Aalpha, PP2AAALPHA, PR65A
protein phosphatase 2, regulatory subunit A, alpha
Homo sapiens
PREY

S100A9

60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14
S100 calcium binding protein A9
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

An integrated workflow for charting the human interaction proteome: insights into the PP2A system.

Glatter T, Wepf A, Aebersold R, Gstaiger M

Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application ... [more]

Mol. Syst. Biol. Jan. 22, 2009; 5(0);237 [Pubmed: 19156129]

Throughput

  • Low Throughput

Curated By

  • BioGRID