YWHAZ
Gene Ontology Biological Process
- Golgi reassembly [IMP]
- RNA metabolic process [TAS]
- apoptotic process [TAS]
- blood coagulation [TAS]
- establishment of Golgi localization [IMP]
- gene expression [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- negative regulation of apoptotic process [TAS]
- platelet activation [TAS]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SIK1
Gene Ontology Biological Process
- cardiac muscle cell differentiation [ISS]
- entrainment of circadian clock by photoperiod [ISS]
- intracellular signal transduction [IDA, ISS]
- negative regulation of CREB transcription factor activity [ISS]
- negative regulation of gluconeogenesis [ISS]
- negative regulation of triglyceride biosynthetic process [ISS]
- positive regulation of anoikis [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA, ISS]
- regulation of cell differentiation [ISS]
- regulation of mitotic cell cycle [ISS]
- regulation of myotube differentiation [ISS]
- regulation of sodium ion transport [ISS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK.
The LKB1 tumour suppressor kinase phosphorylates and activates a number of protein kinases belonging to the AMP-activated protein kinase (AMPK) subfamily. We have used a modified tandem affinity purification strategy to identify proteins that interact with AMPKalpha, as well as the twelve AMPK-related kinases that are activated by LKB1. The AMPKbeta and AMPKgamma regulatory subunits were associated with AMPKalpha, but ... [more]
Throughput
- Low Throughput
Ontology Terms
- hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SIK1 YWHAZ | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 79.3333 | BioGRID | 3482967 | |
| SIK1 YWHAZ | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID