Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs.

Weinmann L, Hoeck J, Ivacevic T, Ohrt T, Muetze J, Schwille P, Kremmer E, Benes V, Urlaub H, Meister G

Small regulatory RNAs including small interfering RNAs (siRNAs) and microRNAs (miRNAs) guide Argonaute (Ago) proteins to specific target RNAs leading to mRNA destabilization or translational repression. Here, we report the identification of Importin 8 (Imp8) as a component of miRNA-guided regulatory pathways. We show that Imp8 interacts with Ago proteins and localizes to cytoplasmic processing bodies (P bodies), structures involved ... [more]

Cell Feb. 06, 2009; 136(3);496-507 [Pubmed: 19167051]

Throughput

  • Low Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007)

Curated By

  • BioGRID