BAIT

ARRB1

1200006I17Rik, AW208571, G430100A01Rik
arrestin, beta 1
GO Process (34)
GO Function (19)
GO Component (12)

Gene Ontology Biological Process

Mus musculus
PREY

SLC9A1

AW554487, Apnh, Nhe1, swe, RP23-32C12.6
solute carrier family 9 (sodium/hydrogen exchanger), member 1
GO Process (33)
GO Function (3)
GO Component (18)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Nedd4-1 and beta-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis, and function.

Simonin A, Fuster D

The ubiquitously expressed mammalian Na(+)/H(+) exchanger 1 (NHE1) controls cell volume and pH but is also critically involved in complex biological processes like cell adhesion, cell migration, cell proliferation, and mechanosensation. Pathways controlling NHE1 turnover at the plasma membrane, however, are currently unclear. Here, we demonstrate that NHE1 undergoes ubiquitylation at the plasma membrane by a process that is unprecedented ... [more]

J. Biol. Chem. Dec. 03, 2010; 285(49);38293-303 [Pubmed: 20855896]

Throughput

  • Low Throughput

Additional Notes

  • beta arrestin cDNA from Imagenes, organism not specified

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLC9A1 ARRB1
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.792BioGRID
2666941

Curated By

  • BioGRID