BAIT

WWM1

YFL010C
WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1
GO Process (0)
GO Function (0)
GO Component (3)

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

PTP1

tyrosine protein phosphatase PTP1, L000001525, YDL230W
Phosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
Kinome Project (Sc)
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins.

Hesselberth JR, Miller JP, Golob A, Stajich JE, Michaud GA, Fields S

BACKGROUND: The WW domain is found in a large number of eukaryotic proteins implicated in a variety of cellular processes. WW domains bind proline-rich protein and peptide ligands, but the protein interaction partners of many WW domain-containing proteins in Saccharomyces cerevisiae are largely unknown. RESULTS: We used protein microarray technology to generate a protein interaction map for 12 of the ... [more]

Genome Biol. Apr. 12, 2006; 7(4);R30 [Pubmed: 16606443]

Throughput

  • High Throughput

Additional Notes

  • A protein microarray was probed with the WW domain from WWM1 (YFL010C).
  • High Throughput: Each WW-domain was used as a probe for two separate protein microarrays. Only those in which four independent interactions were osbserved were considered high-confidence (i.e. significant signals found for proteins printed in duplicate on two separate microarrays).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PTP1 WWM1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2327BioGRID
362189
PTP1 WWM1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3049BioGRID
2092215

Curated By

  • BioGRID