BAIT

ZWINT

HZwint-1, KNTC2AP, ZWINT1
ZW10 interacting kinetochore protein
GO Process (4)
GO Function (2)
GO Component (3)
Homo sapiens
PREY

TRIM17

RBCC, RNF16, terf, RP5-915N17.1
tripartite motif containing 17
GO Process (1)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Terf/TRIM17 stimulates degradation of kinetochore protein ZWINT and regulates cell proliferation.

Endo H, Ikeda K, Urano T, Horie-Inoue K, Inoue S

Terf/TRIM17 is a tripartite motif (TRIM) protein that has been originally isolated from testis. Terf has been characterized to exhibit an E3 ubiquitin ligase activity and to undergo self-ubiquitination. The cellular function of terf and its substrates, however, remain elusive. In the present study, we performed a yeast two-hybrid screening assay using terf as bait and identified a positive clone ... [more]

Unknown Oct. 24, 2011; 0(0); [Pubmed: 22023800]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TRIM17 ZWINT
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID