BAIT

SPT16

CDC68, SSF1, chromatin-remodeling protein SPT16, L000002038, YGL207W

Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; SPT16 specifically required for diauxic shift-induced H2B deposition onto rDNA genes; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome

GO Process (7)

GO Function (3)

GO Component (5)

Gene Ontology Biological Process

DNA replication-independent nucleosome organization [IDA]

DNA-dependent DNA replication [IPI]

chromatin organization involved in regulation of transcription [IMP]

nucleosome assembly [IDA]

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IDA]

positive regulation of transcription initiation from RNA polymerase II promoter [IGI, IMP]

transcription elongation from RNA polymerase II promoter [IPI]

Gene Ontology Molecular Function

chromatin binding [IDA]
histone binding [IDA]
nucleosome binding [IDA]

Gene Ontology Cellular Component

FACT complex [IGI, IPI]

alpha DNA polymerase:primase complex [IDA]

nuclear chromatin [IDA]

replication fork protection complex [IDA]

transcription elongation factor complex [IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SPT5

transcription elongation factor SPT5, L000002031, YML010W

Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair

GO Process (8)

GO Function (7)

GO Component (4)

Gene Ontology Biological Process

7-methylguanosine mRNA capping [IGI, IPI]

intracellular mRNA localization [IMP]

mRNA splicing, via spliceosome [IMP]

negative regulation of transcription elongation from RNA polymerase I promoter [IGI]

positive regulation of transcription elongation from RNA polymerase I promoter [IMP]

positive regulation of transcription elongation from RNA polymerase II promoter [IMP]

regulation of rRNA processing [IMP]

regulation of transcription-coupled nucleotide-excision repair [IGI]

Gene Ontology Molecular Function

RNA polymerase I core binding [IPI]
RNA polymerase I transcription factor binding [IPI]
RNA polymerase II core binding [IPI]
core RNA polymerase I binding transcription factor activity [IC]
core RNA polymerase II binding transcription factor activity [IGI, IMP, IPI]
mRNA binding [IDA]
rRNA binding [IDA]

Gene Ontology Cellular Component

DSIF complex [IPI]

mitochondrion [IDA]

nucleus [IDA]

rDNA heterochromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

FACT, the Bur Kinase Pathway, and the Histone Co-Repressor HirC Have Overlapping Nucleosome-Related Roles in Yeast Transcription Elongation.

Stevens JR, O'Donnell AF, Perry TE, Benjamin JJ, Barnes CA, Johnston GC, Singer RA

Gene transcription is constrained by the nucleosomal nature of chromosomal DNA. This nucleosomal barrier is modulated by FACT, a conserved histone-binding heterodimer. FACT mediates transcription-linked nucleosome disassembly and also nucleosome reassembly in the wake of the RNA polymerase II transcription complex, and in this way maintains the repression of 'cryptic' promoters found within some genes. Here we focus on a ... [more]

PLoS ONE Oct. 25, 2011; 6(10);e25644 [Pubmed: 22022426]

Throughput

Low Throughput

Ontology Terms

phenotype: heat sensitivity (APO:0000147)
phenotype: inviable (APO:0000112)

Additional Notes

at 37 degrees

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SPT5 SPT16	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Evrin C (2022)	Low	-	BioGRID	3547412
SPT16 SPT5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Foltman M (2013)	Low	-	BioGRID	1277089
SPT5 SPT16	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lindstrom DL (2003)	High	-	BioGRID	-
SPT16 SPT5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3597536
SPT5 SPT16	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Evrin C (2022)	Low	-	BioGRID	3547422
SPT16 SPT5	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Han J (2010)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SPT16

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]histone binding [IDA]nucleosome binding [IDA]

Gene Ontology Cellular Component

SPT5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase I core binding [IPI]RNA polymerase I transcription factor binding [IPI]RNA polymerase II core binding [IPI]core RNA polymerase I binding transcription factor activity [IC]core RNA polymerase II binding transcription factor activity [IGI, IMP, IPI]mRNA binding [IDA]rRNA binding [IDA]

Gene Ontology Cellular Component

Synthetic Lethality

Publication

FACT, the Bur Kinase Pathway, and the Histone Co-Repressor HirC Have Overlapping Nucleosome-Related Roles in Yeast Transcription Elongation.

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

chromatin binding [IDA]
histone binding [IDA]
nucleosome binding [IDA]