BAIT

IRE1

ERN1, bifunctional endoribonuclease/protein kinase IRE1, L000000875, YHR079C

Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress

Kinome Project (Sc)

GO Process (6)

GO Function (5)

GO Component (2)

Gene Ontology Biological Process

endoplasmic reticulum unfolded protein response [IMP]

fungal-type cell wall organization [IGI, IMP]

inositol metabolic process [IMP]

protein homooligomerization [IDA]

protein phosphorylation [IDA]

protein trans-autophosphorylation [IDA]

Gene Ontology Molecular Function

endoribonuclease activity [IDA]
protein homodimerization activity [IDA]
protein kinase activity [IDA]
protein serine/threonine kinase activity [IDA]
unfolded protein binding [IDA, IMP]

Gene Ontology Cellular Component

integral component of endoplasmic reticulum membrane [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RPN4

SON1, UFD5, stress-regulated transcription factor RPN4, L000001984, YDL020C

Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress

GO Process (7)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

negative regulation of transcription from RNA polymerase II promoter [IMP]

negative regulation of transcription from RNA polymerase II promoter in response to stress [IMP]

positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IGI, IMP]

positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP]

positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance [IMP]

positive regulation of transcription from RNA polymerase II promoter in response to stress [IEP, IMP]

regulation of DNA repair [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Secretory pathway genes assessed by high-throughput microscopy and synthetic genetic array analysis.

Bircham PW, Maass DR, Roberts CA, Kiew PY, Low YS, Yegambaram M, Matthews J, Jack CA, Atkinson PH

We developed a procedure for automated confocal microscopy to image the effect of the non-essential yeast gene deletion set on the localisation of the plasma membrane GFP-labelled protein Mrh1p-GFP. To achieve this it was necessary to devise an expression system expressing Redstar2 RFP-fluorescence specifically in the nucleus, mCherry RFP at a lower intensity in the cytoplasm and Mrh1p-GFP in the ... [more]

Mol Biosyst Sep. 01, 2011; 7(9);2589-98 [Pubmed: 21731954]

Throughput

High Throughput|Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)
phenotype: colony size (APO:0000063)

Additional Notes

High Throughput: Synthetic Genetic Array (SGA) analysis
Low Throughput: Confirmed by tetrad analysis.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
IRE1 RPN4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Bandyopadhyay S (2010)	High	-10.5203	BioGRID	540939
RPN4 IRE1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3342	BioGRID	364750
IRE1 RPN4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.7703	BioGRID	2127395
RPN4 IRE1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3418	BioGRID	2088823
RPN4 IRE1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Decourty L (2021)	High	-	BioGRID	3395420
IRE1 RPN4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Zheng J (2010)	High	-9.6935	BioGRID	507303
RPN4 IRE1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Pan X (2006)	High	-	BioGRID	454541

Curated By

BioGRID

Interaction Summary

IRE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

endoribonuclease activity [IDA]protein homodimerization activity [IDA]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]unfolded protein binding [IDA, IMP]

Gene Ontology Cellular Component

RPN4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Synthetic Lethality

Publication

Secretory pathway genes assessed by high-throughput microscopy and synthetic genetic array analysis.

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

endoribonuclease activity [IDA]
protein homodimerization activity [IDA]
protein kinase activity [IDA]
protein serine/threonine kinase activity [IDA]
unfolded protein binding [IDA, IMP]

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
sequence-specific DNA binding [IDA]