PREY

TSC1

RP23-362N19.3
tuberous sclerosis 1
GO Process (33)
GO Function (5)
GO Component (12)
Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of 14-3-3epsilon substrates from embryonic murine brain.

Ballif BA, Cao Z, Schwartz D, Carraway KL, Gygi SP

Mice deficient in 14-3-3epsilon exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3epsilon interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3epsilon and ... [more]

J. Proteome Res. Sep. 01, 2006; 5(9);2372-9 [Pubmed: 16944949]

Throughput

  • High Throughput

Curated By

  • BioGRID