STUB1
Gene Ontology Biological Process
- cellular response to misfolded protein [IDA]
- misfolded or incompletely synthesized protein catabolic process [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- positive regulation of chaperone-mediated protein complex assembly [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA]
- protein maturation [TAS]
- protein polyubiquitination [IDA, IMP]
- regulation of glucocorticoid metabolic process [IDA]
- transforming growth factor beta receptor signaling pathway [TAS]
- ubiquitin-dependent SMAD protein catabolic process [IDA]
- ubiquitin-dependent protein catabolic process [IMP]
Gene Ontology Molecular Function- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
Gene Ontology Cellular Component
CYP2E1
Gene Ontology Biological Process
- drug metabolic process [IDA, IMP]
- epoxygenase P450 pathway [IBA]
- exogenous drug catabolic process [IBA]
- heterocycle metabolic process [IDA]
- monoterpenoid metabolic process [IDA]
- oxidation-reduction process [IDA]
- small molecule metabolic process [TAS]
- steroid metabolic process [IMP]
- xenobiotic metabolic process [IBA, TAS]
Gene Ontology Molecular Function- arachidonic acid epoxygenase activity [IBA]
- enzyme binding [IPI]
- heme binding [IDA]
- monooxygenase activity [IDA]
- oxidoreductase activity [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen [TAS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen [IBA]
- oxygen binding [TAS]
- steroid hydroxylase activity [IBA]
- arachidonic acid epoxygenase activity [IBA]
- enzyme binding [IPI]
- heme binding [IDA]
- monooxygenase activity [IDA]
- oxidoreductase activity [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen [TAS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen [IBA]
- oxygen binding [TAS]
- steroid hydroxylase activity [IBA]
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
The ubiquitin-dependent proteasomal degradation (UPD) of human liver cytochrome P450 2E1: identification of sites targeted for phosphorylation and ubiquitination.
Human liver CYP2E1 is a monotopic, endoplasmic reticulum (ER)-anchored cytochrome P450 (P450) responsible for the biotransformation of clinically-relevant drugs, low molecular weight xenobiotics, carcinogens and endogenous ketones. CYP2E1 substrate- complexation converts it into a stable slow-turnover species degraded largely via autophagic lysosomal degradation (ALD). Substrate decomplexation/withdrawal results in a fast-turnover CYP2E1 species, putatively generated through its futile oxidative cycling, that ... [more]
Throughput
- Low Throughput
Additional Notes
- The reaction contained E1, UbcH5a [E2], CHIP [E3], and ubiquitin.
Curated By
- BioGRID