NOS1
Gene Ontology Biological Process
- aging [IEP]
- arginine catabolic process [IDA]
- behavioral response to cocaine [IMP]
- cellular response to epinephrine stimulus [IMP]
- cellular response to growth factor stimulus [ISO]
- cellular response to mechanical stimulus [IMP]
- exogenous drug catabolic process [ISO]
- female pregnancy [IEP]
- multicellular organismal response to stress [ISO]
- muscle contraction [IBA]
- negative regulation of apoptotic process [IMP]
- negative regulation of blood pressure [IMP]
- negative regulation of calcium ion transport [ISO]
- negative regulation of cell proliferation [IMP]
- negative regulation of cytosolic calcium ion concentration [IMP]
- negative regulation of heart contraction [IMP]
- negative regulation of hydrolase activity [ISO]
- negative regulation of insulin secretion [IMP]
- negative regulation of peptidyl-serine phosphorylation [IMP]
- negative regulation of potassium ion transport [ISO]
- negative regulation of serotonin uptake [ISO]
- negative regulation of vasoconstriction [IMP]
- nitric oxide biosynthetic process [IDA, ISO]
- nitric oxide mediated signal transduction [IBA, IMP]
- peptidyl-cysteine S-nitrosylation [IDA, ISO]
- positive regulation of adrenergic receptor signaling pathway involved in heart process [ISO]
- positive regulation of guanylate cyclase activity [IBA]
- positive regulation of histone acetylation [ISO]
- positive regulation of long-term synaptic potentiation [IMP]
- positive regulation of neuron death [IMP]
- positive regulation of sodium ion transmembrane transport [IMP]
- positive regulation of the force of heart contraction [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- positive regulation of vasodilation [IMP, ISO]
- regulation of heart contraction [IMP]
- regulation of sensory perception of pain [IMP]
- regulation of sodium ion transport [ISO]
- response to activity [IEP]
- response to estrogen [IDA]
- response to ethanol [IEP]
- response to heat [IEP, ISO]
- response to hypoxia [IEP, ISO]
- response to lead ion [IEP]
- response to lipopolysaccharide [IEP]
- response to nicotine [IEP]
- response to nitric oxide [IEP]
- response to nutrient levels [IEP]
- response to organic cyclic compound [IEP]
- response to organonitrogen compound [IEP]
- response to peptide hormone [IMP]
- response to vitamin E [IEP]
- striated muscle contraction [ISO]
Gene Ontology Molecular Function- ATPase binding [IPI]
- FMN binding [IDA, TAS]
- NADP binding [IDA, TAS]
- NADPH-hemoprotein reductase activity [IBA]
- amino acid binding [TAS]
- cadmium ion binding [IDA]
- calmodulin binding [IDA]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA, TAS]
- heme binding [IDA, TAS]
- ion channel binding [IPI]
- nitric-oxide synthase activity [IDA, ISO, TAS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- scaffold protein binding [IPI]
- sodium channel regulator activity [IMP]
- ATPase binding [IPI]
- FMN binding [IDA, TAS]
- NADP binding [IDA, TAS]
- NADPH-hemoprotein reductase activity [IBA]
- amino acid binding [TAS]
- cadmium ion binding [IDA]
- calmodulin binding [IDA]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA, TAS]
- heme binding [IDA, TAS]
- ion channel binding [IPI]
- nitric-oxide synthase activity [IDA, ISO, TAS]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- scaffold protein binding [IPI]
- sodium channel regulator activity [IMP]
Gene Ontology Cellular Component
- azurophil granule [IDA]
- cytoplasm [IDA]
- cytoskeleton [ISO]
- cytosol [IDA]
- dendrite [IDA]
- membrane [IDA]
- membrane raft [ISO]
- mitochondrial outer membrane [IDA]
- mitochondrion [IDA]
- nuclear membrane [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- photoreceptor inner segment [IDA]
- plasma membrane [IDA]
- postsynaptic density [IDA]
- protein complex [IDA]
- sarcolemma [IDA, ISO]
- sarcoplasmic reticulum [ISO]
- synapse [IDA, ISO]
- vesicle membrane [IDA]
HSP90AA1
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- chaperone-mediated protein complex assembly [IDA]
- innate immune response [TAS]
- mitochondrial transport [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- protein import into mitochondrial outer membrane [IDA]
- protein refolding [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- response to unfolded protein [NAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Modulation of the heme/substrate-binding cleft of neuronal nitric-oxide synthase regulates binding of Hsp90 and Hsp70 and nNOS Ubiquitination.
Like other nitric-oxide synthase (NOS) enzymes, neuronal NOS (nNOS) turnover and activity are regulated by the Hsp90/Hsp70-based chaperone machinery, which regulates signaling proteins by modulating ligand binding clefts (Pratt, W.B., Morishima, Y., and Osawa, Y. (2008) J. Biol. Chem. 283, 22855-22889). We have previously shown that nNOS turnover is due to Hsp70/CHIP-dependent ubiquitination and proteasomal degradation. In this ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID