BAIT

PPP1CB

HEL-S-80p, PP-1B, PP1B, PP1beta, PPP1CD
protein phosphatase 1, catalytic subunit, beta isozyme
Alzheimer's Disease Project
Cell Cycle - Mitotic Spindle Assembly Project
Homo sapiens
PREY

NONO

NMT55, NRB54, P54, P54NRB, PPP1R114
non-POU domain containing, octamer-binding
Fanconi Anemia Project
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Consensus PP1 binding motifs regulate transcriptional corepression and alternative RNA splicing activities of the steroid receptor coregulators, p54nrb and PSF.

Liu L, Xie N, Rennie P, Challis JR, Gleave M, Lye SJ, Dong X

Originally identified as essential pre-mRNA splicing factors, non-POU-domain-containing, octamer binding protein (p54nrb) and PTB-associated RNA splicing factor (PSF) are also steroid receptor corepressors. The mechanisms by which p54nrb and PSF regulate gene transcription remain unclear. Both p54nrb and PSF contain protein phosphatase 1 (PP1) consensus binding RVxF motifs, suggesting that PP1 may regulate phosphorylation status of p54nrb and PSF and ... [more]

Mol. Endocrinol. Jul. 01, 2011; 25(7);1197-210 [Pubmed: 21566083]

Throughput

  • Low Throughput

Curated By

  • BioGRID