Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Cohesin-RAD21 Interactome.

Panigrahi AK, Zhang N, Otta SK, Pati D

The cohesin complex holds the sister chromatids together from S phase until the metaphase-to-anaphase transition and ensures both their proper cohesin and timely separation. In addition to its canonical function in chromosomal segregation, cohesion has been suggested by several lines of investigation in recent years to play additional roles in apoptosis, DNA damage response, transcriptional regulation, and hematopoiesis. To better ... [more]

Unknown Dec. 07, 2011; 0(0); [Pubmed: 22145905]

Throughput

  • High Throughput

Ontology Terms

  • cell line: molt-4 cell (BTO:0000873) [acute t cell leukemia (DOID:5603)]

Curated By

  • BioGRID