BAIT

RAD21

CDLS4, HR21, HRAD21, MCD1, NXP1, SCC1, hHR21
RAD21 homolog (S. pombe)
Cell Cycle - Mitotic Spindle Assembly Project
Fanconi Anemia Project
Homo sapiens
PREY

FHL3

SLIM2
four and a half LIM domains 3
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A Cohesin-RAD21 Interactome.

Panigrahi AK, Zhang N, Otta SK, Pati D

The cohesin complex holds the sister chromatids together from S phase until the metaphase-to-anaphase transition and ensures both their proper cohesin and timely separation. In addition to its canonical function in chromosomal segregation, cohesion has been suggested by several lines of investigation in recent years to play additional roles in apoptosis, DNA damage response, transcriptional regulation, and hematopoiesis. To better ... [more]

Unknown Dec. 07, 2011; 0(0); [Pubmed: 22145905]

Throughput

  • High Throughput

Ontology Terms

  • molt-4 cell (BTO:0000873) [acute t cell leukemia (DOID:5603)]

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAD21 FHL3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
FHL3 RAD21
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID