BAIT

CKA1

casein kinase 2 catalytic subunit CKA1, L000000343, YIL035C
Alpha catalytic subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

DAM1

L000004420, YGR113W
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
Saccharomyces cerevisiae (S288c)

Biochemical Activity (Phosphorylation)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Overlapping Kinetochore Targets of CK2 and Aurora B Kinases in Mitotic Regulation.

Peng Y, Wong CC, Nakajima Y, Tyers RG, Sarkeshik AS, Yates J, Drubin DG, Barnes G

Protein kinase CK2 is one of the most conserved kinases in eukaryotic cells and plays essential roles in diverse processes. Despite its roles in cell division, our understanding of how CK2 regulates cell cycle progression is limited. In this study, we revealed a regulatory role for CK2 in kinetochore function. The kinetochore is a multi-protein complex that assembles on the ... [more]

Unknown Jun. 01, 2011; 0(0); [Pubmed: 21633108]

Throughput

  • Low Throughput

Additional Notes

  • Ndc10 is phosphorylated in vitro by the casein kinase complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DAM1 CKA1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2773BioGRID
1985131

Curated By

  • BioGRID