BAIT
PRKCA
AI875142, Pkca, RP23-145G23.2
protein kinase C, alpha
GO Process (51)
GO Function (7)
GO Component (18)
Gene Ontology Biological Process
- cellular calcium ion homeostasis [IMP]
- cellular response to carbohydrate stimulus [IDA]
- central nervous system neuron axonogenesis [ISO]
- chondrocyte differentiation [IDA]
- desmosome assembly [ISO]
- histone H3-T6 phosphorylation [ISO]
- inactivation of MAPK activity [IMP]
- induction of positive chemotaxis [IMP]
- intracellular signal transduction [ISO]
- intrinsic apoptotic signaling pathway [IMP]
- learning or memory [ISO]
- negative regulation of cell proliferation [IGI]
- negative regulation of glial cell apoptotic process [ISO]
- negative regulation of glucose import [IMP]
- negative regulation of heart contraction [ISO]
- negative regulation of insulin receptor signaling pathway [IMP]
- negative regulation of protein kinase activity [IMP]
- negative regulation of protein phosphorylation [IGI, IMP]
- negative regulation of translation [ISO]
- neutrophil chemotaxis [IMP]
- peptidyl-serine autophosphorylation [IDA]
- peptidyl-serine phosphorylation [ISO]
- peptidyl-threonine phosphorylation [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of angiogenesis [ISO]
- positive regulation of blood vessel endothelial cell migration [ISO]
- positive regulation of cardiac muscle hypertrophy [ISO]
- positive regulation of cell adhesion [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of dense core granule biogenesis [IMP]
- positive regulation of endothelial cell migration [ISO]
- positive regulation of endothelial cell proliferation [ISO]
- positive regulation of exocytosis [ISO]
- positive regulation of inflammatory response [IMP]
- positive regulation of lipopolysaccharide-mediated signaling pathway [ISO]
- positive regulation of macrophage differentiation [IMP]
- positive regulation of mitotic cell cycle [ISO]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of synapse assembly [ISO]
- protein autophosphorylation [ISO]
- protein phosphorylation [IDA, ISO]
- regulation of muscle contraction [IMP]
- regulation of peptidyl-tyrosine phosphorylation [IMP]
- regulation of platelet aggregation [IMP, ISO]
- regulation of receptor-mediated endocytosis [ISO]
- regulation of the force of heart contraction [IMP]
- response to estradiol [ISO]
- response to ethanol [ISO]
- response to interleukin-1 [ISO]
- response to reactive oxygen species [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical part of cell [IDA]
- cell [IMP]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- dendrite [IDA]
- endoplasmic reticulum [ISO]
- extracellular vesicular exosome [ISO]
- intracellular [IMP]
- membrane [IDA]
- membrane raft [ISO]
- mitochondrion [IDA, ISO]
- neuron projection [ISO]
- neuronal cell body [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [ISO]
- photoreceptor outer segment [IDA]
- plasma membrane [IDA, ISO]
- protein complex [ISO]
Mus musculus
PREY
ITGB3
CD61, GP3A, INGRB3, RP24-460H24.1
integrin beta 3
GO Process (45)
GO Function (11)
GO Component (18)
Gene Ontology Biological Process
- activation of protein kinase activity [ISO]
- apolipoprotein A-I-mediated signaling pathway [ISO]
- cell adhesion [ISO]
- cell growth [ISO]
- cell migration [ISO]
- cell projection morphogenesis [IC]
- cell-matrix adhesion [IGI, ISO]
- cell-substrate adhesion [ISO]
- cell-substrate junction assembly [IDA]
- cellular calcium ion homeostasis [ISO]
- heterotypic cell-cell adhesion [ISO]
- integrin-mediated signaling pathway [ISO]
- negative chemotaxis [ISO]
- negative regulation of cell death [ISO]
- negative regulation of lipid storage [ISO]
- negative regulation of lipid transport [ISO]
- negative regulation of lipoprotein metabolic process [ISO]
- negative regulation of low-density lipoprotein particle receptor biosynthetic process [ISO]
- negative regulation of macrophage derived foam cell differentiation [ISO]
- platelet activation [ISO]
- platelet aggregation [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of MAPK cascade [ISO]
- positive regulation of adenylate cyclase-inhibiting opioid receptor signaling pathway [ISO]
- positive regulation of angiogenesis [ISO]
- positive regulation of bone resorption [IC, ISO]
- positive regulation of cell adhesion mediated by integrin [ISO]
- positive regulation of cell migration [IMP]
- positive regulation of endothelial cell migration [ISO]
- positive regulation of endothelial cell proliferation [ISO]
- positive regulation of fibroblast migration [ISO]
- positive regulation of glomerular mesangial cell proliferation [ISO]
- positive regulation of leukocyte migration [ISO]
- positive regulation of osteoblast proliferation [IMP]
- positive regulation of osteoclast differentiation [IC]
- positive regulation of peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of smooth muscle cell migration [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- protein folding [ISO]
- regulation of G-protein coupled receptor protein signaling pathway [ISO]
- regulation of cell migration [IMP]
- regulation of release of sequestered calcium ion into cytosol [ISO]
- smooth muscle cell migration [ISO]
- substrate adhesion-dependent cell spreading [ISO]
- viral entry into host cell [ISO]
Gene Ontology Molecular Function- cell adhesion molecule binding [ISO]
- extracellular matrix binding [ISO]
- fibrinogen binding [ISO]
- fibronectin binding [ISO]
- identical protein binding [ISO]
- integrin binding [IPI]
- peptide binding [ISO]
- protease binding [ISO]
- protein binding [IPI]
- protein disulfide isomerase activity [ISO]
- vascular endothelial growth factor receptor 2 binding [ISO]
- cell adhesion molecule binding [ISO]
- extracellular matrix binding [ISO]
- fibrinogen binding [ISO]
- fibronectin binding [ISO]
- identical protein binding [ISO]
- integrin binding [IPI]
- peptide binding [ISO]
- protease binding [ISO]
- protein binding [IPI]
- protein disulfide isomerase activity [ISO]
- vascular endothelial growth factor receptor 2 binding [ISO]
Gene Ontology Cellular Component
- alpha9-beta1 integrin-ADAM8 complex [IDA]
- apical plasma membrane [IDA, ISO]
- cell surface [ISO]
- external side of plasma membrane [IDA]
- extracellular vesicular exosome [ISO]
- filopodium membrane [ISO]
- focal adhesion [ISO]
- integrin alpha9-beta1 complex [TAS]
- integrin alphav-beta3 complex [ISO]
- integrin complex [ISO]
- lamellipodium membrane [ISO]
- melanosome [ISO]
- membrane [ISO]
- microvillus membrane [ISO]
- nucleus [ISO]
- plasma membrane [ISO]
- receptor complex [ISO]
- ruffle membrane [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A novel protein kinase C alpha-dependent signal to ERK1/2 activated by alphaVbeta3 integrin in osteoclasts and in Chinese hamster ovary (CHO) cells.
We identified a novel protein kinase C (PKC)alpha-dependent signal to extracellular signal-regulated kinase (ERK)1/2 in mouse osteoclasts and Chinese hamster ovary (CHO) cells, specifically activated by the alphaVbeta3 integrin. It involves translocation (i.e. activation) of PKCalpha from the cytosol to the membrane and/or the Triton X-100-insoluble subcellular fractions, with recruitment into a complex with alphaVbeta3 integrin, growth factor receptor-bound protein ... [more]
J. Cell. Sci. Aug. 01, 2005; 118(0);3263-75 [Pubmed: 16014375]
Throughput
- Low Throughput
Curated By
- BioGRID